Lycopene-epsilon-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck).
Identifieur interne : 002704 ( Main/Exploration ); précédent : 002703; suivant : 002705Lycopene-epsilon-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck).
Auteurs : Neng-Guo Tao [République populaire de Chine] ; Juan Xu ; Yun-Jiang Cheng ; Xiu-Xin DengSource :
- Biotechnology letters [ 0141-5492 ] ; 2005.
English descriptors
- KwdEn :
- Alternative Splicing, Amino Acid Sequence, Base Sequence, Citrus sinensis (enzymology), Citrus sinensis (genetics), Cloning, Molecular, DNA, Complementary (chemistry), DNA, Complementary (genetics), Intramolecular Lyases (genetics), Molecular Sequence Data, Mutagenesis, Insertional, Mutation, Plant Proteins (genetics), RNA Precursors (genetics), Sequence Analysis, DNA, Sequence Deletion.
- MESH :
- chemical , chemistry : DNA, Complementary.
- enzymology : Citrus sinensis.
- genetics : Citrus sinensis, DNA, Complementary, Intramolecular Lyases, Plant Proteins, RNA Precursors.
- Alternative Splicing, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Mutagenesis, Insertional, Mutation, Sequence Analysis, DNA, Sequence Deletion.
Abstract
Lycopene-epsilon-cyclase is one of the key enzymes related to alpha-carotene metabolism in plants. A full-length cDNA of 1300 bp encoding lycopene-epsilon-cyclase (Lyce) was generated from Cara Cara navel orange, a unique navel orange containing both lycopene and beta-carotene in its pulp, with little or no alpha-carotene. The gene had a 14 bp nucleotides deletion and caused a terminal mutation. DNA sequence corresponding to the deletion region revealed that two repeats of 6 bp (AGGTGT) were flanking the region in both Cara Cara and its original variety, Washington navel oranges, but a 2 bp (AT) insertion was only found in Cara Cara which explain the alternative splicing character of the gene.
DOI: 10.1007/s10529-005-5793-8
PubMed: 16086259
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lycopene-epsilon-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck).</title><author><name sortKey="Tao, Neng Guo" sort="Tao, Neng Guo" uniqKey="Tao N" first="Neng-Guo" last="Tao">Neng-Guo Tao</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Juan" sort="Xu, Juan" uniqKey="Xu J" first="Juan" last="Xu">Juan Xu</name></author><author><name sortKey="Cheng, Yun Jiang" sort="Cheng, Yun Jiang" uniqKey="Cheng Y" first="Yun-Jiang" last="Cheng">Yun-Jiang Cheng</name></author><author><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16086259</idno><idno type="pmid">16086259</idno><idno type="doi">10.1007/s10529-005-5793-8</idno><idno type="wicri:Area/PubMed/Corpus">000C98</idno><idno type="wicri:Area/PubMed/Curation">000C98</idno><idno type="wicri:Area/PubMed/Checkpoint">000C98</idno><idno type="wicri:Area/Ncbi/Merge">000407</idno><idno type="wicri:Area/Ncbi/Curation">000407</idno><idno type="wicri:Area/Ncbi/Checkpoint">000407</idno><idno type="wicri:doubleKey">0141-5492:2005:Tao N:lycopene:epsilon:cyclase</idno><idno type="wicri:Area/Main/Merge">002818</idno><idno type="wicri:Area/Main/Curation">002704</idno><idno type="wicri:Area/Main/Exploration">002704</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lycopene-epsilon-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck).</title><author><name sortKey="Tao, Neng Guo" sort="Tao, Neng Guo" uniqKey="Tao N" first="Neng-Guo" last="Tao">Neng-Guo Tao</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Juan" sort="Xu, Juan" uniqKey="Xu J" first="Juan" last="Xu">Juan Xu</name></author><author><name sortKey="Cheng, Yun Jiang" sort="Cheng, Yun Jiang" uniqKey="Cheng Y" first="Yun-Jiang" last="Cheng">Yun-Jiang Cheng</name></author><author><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="ISSN">0141-5492</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alternative Splicing</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Citrus sinensis (enzymology)</term><term>Citrus sinensis (genetics)</term><term>Cloning, Molecular</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (genetics)</term><term>Intramolecular Lyases (genetics)</term><term>Molecular Sequence Data</term><term>Mutagenesis, Insertional</term><term>Mutation</term><term>Plant Proteins (genetics)</term><term>RNA Precursors (genetics)</term><term>Sequence Analysis, DNA</term><term>Sequence Deletion</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>DNA, Complementary</term><term>Intramolecular Lyases</term><term>Plant Proteins</term><term>RNA Precursors</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alternative Splicing</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Molecular Sequence Data</term><term>Mutagenesis, Insertional</term><term>Mutation</term><term>Sequence Analysis, DNA</term><term>Sequence Deletion</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lycopene-epsilon-cyclase is one of the key enzymes related to alpha-carotene metabolism in plants. A full-length cDNA of 1300 bp encoding lycopene-epsilon-cyclase (Lyce) was generated from Cara Cara navel orange, a unique navel orange containing both lycopene and beta-carotene in its pulp, with little or no alpha-carotene. The gene had a 14 bp nucleotides deletion and caused a terminal mutation. DNA sequence corresponding to the deletion region revealed that two repeats of 6 bp (AGGTGT) were flanking the region in both Cara Cara and its original variety, Washington navel oranges, but a 2 bp (AT) insertion was only found in Cara Cara which explain the alternative splicing character of the gene.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Cheng, Yun Jiang" sort="Cheng, Yun Jiang" uniqKey="Cheng Y" first="Yun-Jiang" last="Cheng">Yun-Jiang Cheng</name><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name><name sortKey="Xu, Juan" sort="Xu, Juan" uniqKey="Xu J" first="Juan" last="Xu">Juan Xu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tao, Neng Guo" sort="Tao, Neng Guo" uniqKey="Tao N" first="Neng-Guo" last="Tao">Neng-Guo Tao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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